Investigation of the mechanisms of Angelica dahurica root extract-induced vasorelaxation in isolated rat aortic rings
نویسندگان
چکیده
BACKGROUND The root of Angelica dahurica Bentham et Hooker (Umbelliferae) has been used as a traditional medicine for colds, headache, dizziness, toothache, supraorbital pain, nasal congestion, acne, ulcer, carbuncle, and rheumatism in China, Japan, and Korea. Interestingly, it has been used in the treatment of vascular diseases including hypertension. The aim of this study was to provide pharmacological evidence for the anti-hypertensive effect of A. dahurica by investigating the mechanism underlying its vasorelaxant effect. METHODS The vasorelaxant effects of a 70% methanol extract of the A. dahurica root (ADE) on rat thoracic aorta and its underlying mechanisms were assessed. Isolated rat aortic rings were suspended in organ chambers containing 10 ml Krebs-Henseleit (K-H) solution and placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system. RESULTS ADE causes concentration-dependent relaxation in both endothelium-intact and endothelium-denuded aortic rings precontracted with phenylephrine (PE; 1 μM) or potassium (KCl; 60 mM) in K-H solution. And pre-treatment with ADE (1 mg/ml) inhibited calcium-induced vasocontraction of aortic rings induced by PE or KCl. However, ADE pre-treatment did not affect the contraction induced by PE or caffeine in Ca(2+)-free K-H solution. CONCLUSIONS These results suggested that the ADE has vasorelaxant effect and the vasorelaxant activity is mediated by endothelium-independent pathway that includes the blockade of extracellular calcium influx through the receptor-operated Ca(2+) channel and voltage-dependent calcium channel pathways.
منابع مشابه
Author's response to reviews Title:Investigation of the mechanisms of Angelica dahurica root extract-induced vasorelaxation in isolated rat aortic rings Authors:
متن کامل
Saffron Induced Relaxation in Isolated Rat Aorta via Endothelium Dependent and Independent Mechanisms
Crocus sativus L. (saffron) is a widely used food additive for its color and taste. The hypotensive effects of saffron have been shown in previous studies. The aim of this study was to evaluate the mechanism of vasodilatory effects induced by saffron on isolated rat aorta.To study the vasodilatory effects of saffron aqueous extract (0.5, 1 and 2 mg/mL), isolated rat thoracic aorta rings were co...
متن کاملSaffron Induced Relaxation in Isolated Rat Aorta via Endothelium Dependent and Independent Mechanisms
Crocus sativus L. (saffron) is a widely used food additive for its color and taste. The hypotensive effects of saffron have been shown in previous studies. The aim of this study was to evaluate the mechanism of vasodilatory effects induced by saffron on isolated rat aorta.To study the vasodilatory effects of saffron aqueous extract (0.5, 1 and 2 mg/mL), isolated rat thoracic aorta rings were co...
متن کاملVasorelaxant effects of Angelica decursiva root on isolated rat aortic rings
BACKGROUND Hypertension is one of the most important risk factors for cardiovascular disease (CVD) and a worldwide problem. Despite increases in the development of synthetic drugs for hypertension treatment, the rate of untreated and uncontrolled hypertension remains high. These drugs are effective, but can also cause side effects. Approximately 80% of the world population uses herbal medicines...
متن کاملEffect of Subchronic Administration of Aqueous Artemisia annua Extract on a1-Adrenoceptor Agonist-Induced Contraction of Isolated Aorta in Rat
Artemisia species, growing in almost all the northern hemisphere, is used in folk medicine of some countries as a remedy for hypertension. Since some cardiovascular disorders including hypertension are accompanied with altered responsiveness of vascular alpha-adrenergic receptors, the effect of aqueous Artemisia annua extract (100 and 200 mg kg-1) on a1-adrenoceptor agonist-induced contraction ...
متن کامل